Figure 2. Unrooted 16S rRNA gene tree based on maximum likelihood analyses of bacteria associated with Nitocra spinipes.

Treatments: ciprofloxacin (orange), sulfomethoxazole (blue), trimethoprim (red); Controls: SS (grey), acetone (purple) and DMSO (green). Reference sequences are in black. Sequences are presented with their GenBank accession numbers followed by identity descriptions. Number of 16S rRNA gene sequences identified in each clone library is indicated in brackets following the clone number. Identical sequences found within a clone library were not included in the tree. One thousand bootstrapped replicate resampled datasets were analyzed. Bootstrap values are indicated as percentage and not shown if below 50%.