Table 1. Relative diversity of bacterial 16S rRNA gene clones associated with Nitocra spinipes in antibiotics and control treatments.
Organism | Frequency, %, and number of clones in each library | |||||
(closest match NCBI, its % identity and GenBank Accession No) | Antibiotic treatments | Controls | ||||
Cipr | Sulf | Trim | SS | Ac | DMSO | |
1. Unc. Hydrogenophaga sp. | 7 | 7 | 10 | 50 | 60 | 63 |
(99%, FR691429) | (2) | (2) | (3) | (15) | (18) | (19) |
2. Unc. Acidovorax sp. | nd | nd | nd | 20 | 10 | 13 |
(99%, HM027578) | (6) | (3) | (4) | |||
3. Unc. Betaproteobacterium sp. | nd | nd | nd | 7 | 7 | 7 |
(99%, AB626830) | (2) | (2) | (2) | |||
4. Unc. Bacteroidetes bacterium | nd | nd | nd | 10 | 7 | nd |
(92%, FJ752808) | (3) | (2) | ||||
5. Unc. Cyanobacterium sp. | nd | nd | nd | 13 | 17 | 17 |
(99%, FJ 839359) | (14) | (5) | (5) | |||
6. Cardinium endosymbiont | 80 | 67 | 73 | nd | nd | nd |
(93–94%, AB506778) | (24) | (20) | (22) | |||
7. Unc. Phyllobacteriaceae sp. | 7 | 13 | 7 | nd | nd | nd |
(99%, AM403238) | (2) | (14) | (2) | |||
8. Unc. Achromobacter sp. | 7 | 13 | 10 | nd | nd | nd |
(99%, FM626830) | (2) | (14) | (3) |
Unc., Uncultured; nd, 16S rRNA sequence not detected; Treatments: ciprofloxacin (Cipr), Sulfomethoxazole (Sulf), trimethoprim (Trim); Controls: synthetic seawater (SS), acetone (Ac), dimethylsulfoxid (DMSO). Percentages are rounded to the nearest whole number and the corresponding number of clones per library is given in brackets.