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To measure the width of individual hyper-reflective bundles, 3–8 bundles were randomly chosen from 1 AO-SLO image (1.5°×1.5°). The digital caliper tool was used to measure the width at a minimum of 3 points in 1 bundle by 2 independent experienced graders (i.e., the bundle width of this area was defined as the mean width of 18 points).
