Figure 5. Bacterial loads and pathological changes in the lungs and spleens of SchuS4 infected mice following antibiotic therapy that succumb to infection.

Mice were infected intranasally with 50 CFU SchuS4. Three days after infection mice were treated once daily with 5 mg/kg LVF diluted in 5% dextrose water intraperitoneally for 14 days. After antibiotic treatment, mice (n = 4) showing irreversible signs of illness were euthanized. Lungs and spleens were collected and assessed for bacterial loads (A) or pathological changes (B–E). Representative photomicrographs for each time point are shown. Plates B and D are at 2× magnification and plates C and E are 40× magnifications of the areas indicated by the arrow on the corresponding 2× plates.