Figure 4. Activation of MKP1 and c-Fos signaling by C. albicans Δals mutants.

C. albicans yeasts were added to TR146 oral epithelial cell monolayers and incubated under standard conditions for 2 h. Results from a fungal:epithelial cell MOI of 10 are shown here. Epithelial cell lysates were separated by SDS-PAGE and Western blotted to detect MKP1, c-Fos or α-actin (positive control). Data are representative of four independent experiments. There was no difference in MKP1 or c-Fos between control C. albicans strains (SC5314 and CAI4) and the Δals mutants.