| TUV 93-0 |
EHEC O157:H7 strain EDL933; stx
− derivative |
[49]
|
| ZAP198 |
EHEC O157:H7 strain NCTC 12900 stx
−
|
[50]
|
| ZAP198Δler
|
Unmarked ler deletion mutant in strain ZAP198 |
[50]
|
| ZAP198ΔgrlA-Tn-Kan |
grlA transposon mutant in EHEC strain ZAP198 (Tn5) |
[51]
|
| BW25113 |
E. coli K-12. |
[52]
|
| BW25113 rpos:Kan |
BW25113 Keio mutant of rpoS. |
[52]
|
| MC1000 |
araD139 Δ(araABC-leu)7679 galU galK Δ(lac)X74 rpsL thi. |
[53]
|
| VS184 |
MC1000ΔqseC
|
[54]
|
| XL1-Blue |
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI
qZΔM15 Tn10 (Tetr)] |
Stratagene |
|
Plasmid
|
|
|
| pAJR70 |
pACYC eGFP |
[30]
|
| pAHE8 |
pAJR70 Ω [BamHI KpnI −531 bpNleH1_291::gfp] |
This study |
| pAHE18 |
pAJR70 Ω [BamHI KpnI −120 bpNleH1_291::gfp] |
This study |
| pAHE19 |
pAJR70 Ω [BamHI KpnI −283 bpNleH1_291::gfp] |
This study |
| pAHE20 |
pAJR70 Ω [BamHI KpnI −113 bpNleH2_293::gfp] |
This study |
| pAHE21 |
pAJR70 Ω [BamHI KpnI −291 bpNleH2_293::gfp] |
This study |
| pAHE22 |
pAJR70 Ω [BamHI KpnI −655 bpNleH2_293::gfp] |
This study |
| pAJR75 |
pAJR70 Ω [BamHI KpnI −442 bp including LEE5 promoter cloned in frame 5′ to egfp
|
Roe 2004 |
| pAJR145 |
pACYC rpsm::GFP+ |
Roe 2004 |
| placZ
|
β-galactosidase enzyme constitutively produced by mammalian expression vector |
Stratagene |
| NF-κB luc
|
Firefly luciferase gene under the control of canonical NF-κB promoter |
Stratagene |
| pCMVTag3A |
N-terminal myc tagging mammalian expression vector |
Stratagene |
| pCMV-NleH1 |
pCMVTag3A Ω [PstI HindIII - NleH1 ORF] |
This study |
| pCMV-NleH2 |
pCMVTag3A Ω [PstI HindIII – NleH2 ORF] |
This study |
| pCMV-NleH1(D258) |
Site directed mutant (SDM) of NleH1 residue D258 to alanine (A) |
This study |
| pCMV-NleH1(E173) |
SDM of NleH1 residue E173 to alanine (A) |
This study |
| pCMV-NleH1(K159A) |
SDM of NleH1 residue K159 to alanine (A) |
This study |
| pJ201-OspG |
OspG ORF with PstI+HindIII sites |
DNA2.0/this study |
| pCMV-OspG |
pCMVTag3A Ω [PstI HindIII - OspG ORF] |
This study |
