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. 2012 Mar 12;7(3):e33368. doi: 10.1371/journal.pone.0033368

Figure 1. Generation and confirmation of bone-specific Kif3a conditional knockout mice.

Figure 1

(A) A typical agarose gel resulting from PCR genotyping of genomic DNA from tail biopsies of transgenic mice. Bands indicate floxed (490 bp) and wild-type (360 bp) Kif3a and Cre recombinase (650 bp). 18S (870 bp) used as a positive control in the Cre PCR reactions. Floxed and recombined Kif3a allele present in Colα1(I) 2.3-Cre;Kif3afl/fl mice due to heterogeneous tissue in tail biopsies. (B) To assess Cre specificity, Colα1(I) 2.3-Cre mice were crossed with Rosa26R reporter mice. Effective Cre recombination was detected by LacZ staining in osteoblasts and osteocytes of Colα1(I) 2.3-Cre;R26R mice (right) but not in littermates lacking Cre (left). LacZ staining was not visible in muscle tissue of Colα1(I) 2.3-Cre;R26R mice. (b- bone, bm- bone marrow, m- muscle).