Table 1. Primer sequences and PCR-RFLP conditions for amplification of RECK SNPs.
SNP | Sequences | Product | Enzyme |
RECKrs16932912 | 5′-TGGAGATTGTTGATGGTCTC-3 5′-CGGTACACAATGCTCAATAC-3′ | G/G: 353 bpA/A: 250 bp, 103 bp | TfiI |
RECKrs11788747 | 5′-GTAGAAGAAGTGACTCATCC-3 5′-ATCTCACTCCGAAGATAACC-3′ | A/A: 242 bpG/G: 140 bp, 102 bp | RsaI |
RECKrs10972727 | 5′-TTCTGTCAGGTCATGGAACA-3′ 5′-TGCAGTTAAGACTGGAGAAG-3′ | T/T: 224 bpA/A: 119 bp, 105 bp | HpyCH4IV |