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. 2012 Mar 12;7(3):e33517. doi: 10.1371/journal.pone.0033517

Table 1. Primer sequences and PCR-RFLP conditions for amplification of RECK SNPs.

SNP Sequences Product Enzyme
RECKrs16932912 5′-TGGAGATTGTTGATGGTCTC-3 5′-CGGTACACAATGCTCAATAC-3′ G/G: 353 bpA/A: 250 bp, 103 bp TfiI
RECKrs11788747 5′-GTAGAAGAAGTGACTCATCC-3 5′-ATCTCACTCCGAAGATAACC-3′ A/A: 242 bpG/G: 140 bp, 102 bp RsaI
RECKrs10972727 5′-TTCTGTCAGGTCATGGAACA-3′ 5′-TGCAGTTAAGACTGGAGAAG-3′ T/T: 224 bpA/A: 119 bp, 105 bp HpyCH4IV