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. 2012 Mar 12;7(3):e33546. doi: 10.1371/journal.pone.0033546

Figure 2. DSS-induced colitis alters mRNA expression of L-Arg metabolic pathway genes.

Figure 2

7-week-old C57BL/6 mice received 4% DSS in the drinking water or water alone (Ctrl) for 7 days. At sacrifice on day 7, the colon was removed and tissues were obtained for RNA isolation and assessment of gene expression by real-time PCR. (A–G) Tissue mRNA expression of L-Arg metabolic pathway genes: L-Arg transporters CAT1 (A) and CAT2 (B), metabolic enzymes iNOS (C), Arg1 (D), and Arg2 (E), as well as the downstream enzymes ODC (F) and OAT (G). n = 4–10 per group. *p<0.05, ***p<0.001 vs. control.