Figure 5. Effect of DSS treatment and L-Arg supplementation on L-Arg metabolic pathways.
Mice received 4% DSS ± 1% L-Arg in the drinking water as in Figures 3–4. (A) At sacrifice on day 10, a piece of colon was obtained for RNA isolation or polyamine determination by HPLC. (A–G) Tissue mRNA levels of the genes indicated, as assessed by real-time PCR. (H) Tissue polyamine levels were assessed by HPLC as described in the Methods. n = 4–15 per group. *p<0.05, **p<0.01, ***p<0.001 vs. control. §p<0.05 vs. DSS.