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. Author manuscript; available in PMC: 2013 Feb 24.
Published in final edited form as: Immunity. 2012 Feb 24;36(2):239–250. doi: 10.1016/j.immuni.2011.12.015

Figure 3. IL-6 induced phosphorylation of STAT3 is prolonged in the presence of JAK1GQM-DVP.

Figure 3

(A) Kinase inhibition assays were performed using the kinase domain of JAK1 and JAK1GQM-DVP. JAK1GQM-DVP was not inhibited by SOCS3. Error bars represent +/− range from two experiments. Data were normalized to no-inhibitor controls.

(B) Wild-type and mutant (GQM-DVP) JAK1 constructs were transfected into JAK1−/− (U4A) cells. Cells were stimulated with IL-6 plus s-IL-6Rα for 15 min and then harvested at the indicated times. Lysates were analyzed by immunoblotting with antibodies specific for phosphorylated STAT3, total STAT3, SOCS3 and JAK1.