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. Author manuscript; available in PMC: 2013 Feb 24.
Published in final edited form as: Immunity. 2012 Feb 24;36(2):239–250. doi: 10.1016/j.immuni.2011.12.015

Figure 4. NMR analysis identifies the surface of SOCS3 that interacts with JAK2.

Figure 4

(A) Ribbon diagram of the structure of SOCS322–185 (PDB ID 2HMH) with important secondary structural motifs indicated. The dashed line indicates the first seven residues of the KIR which are unstructured in the absence of JAK.

(B) 1H-15N and 1H-13C HMQC analysis of the SOCS3-JAK2JH1 interaction are shown in upper and lower panels respectively. The SOCS spectra are in red and the SOCS3-JAK2JH1 spectrum in black.

(C) Surface diagram of SOCS3. Residues whose resonances shift in the presence of JAK2JH1 highlighted in red. The orientation of SOCS3 on the left is identical to that in (A).