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MCD features of ferric holo-Rv0203-notag compared to selected five-coordinate, oxygen ligated heme molecules.
| Protein (ref.) | Ligand | Ox. State | Soret/nm (Δε/H/(mM cm T)-1) | Visible/nm (Δε/H/(mM cm T)-1) |
|---|---|---|---|---|
| Rv0203-notag (this work) | O | Fe(III) | 398 (6.0) | 527 (0), 627 (-4.2) |
| H93Y-Mb (pH 5.1-10.5) (38) | Tyr | Fe(III) | 398 (13.2) | 528 (-1.34), 621 (-10.9) |
| H93G-Mb (pH 10) (38) | OH- | Fe(III) | 397 (13.9) | 534 (-1.94), 621 (-5.21) |
| Fe-PPIXDME + CH3O- (38) | CH3O- | Fe(III) | 398 (13.7) | 530 (-2.28), 618 (12.8) |
