. Author manuscript; available in PMC: 2013 Feb 21.

Published in final edited form as: Biochemistry. 2012 Feb 8;51(7):1518–1531. doi: 10.1021/bi2018305

Table 5.

Off-rates and k_obs for the Rv0203-notag mutants compared to WT-Rv0203-notag.

Rv0203-notag mutant	Single Exp. k_-heme (s^-1)	Double Exp. k_f and k_s (s^-1)	k_obs (s^-1)
Y59F	0.094 ± 0.02	k_f: 1.2 ± 0.5 (15% ± 5) k_s: 0.07 ± 0.0	87 ± 17
Y59H	0.1 ± 0.03	k_f: 0.95 (30%), k_s: 0.06	82 ± 37
Y59A	0.367 ± 0.069	k_f: 2.15 ± 0.78 (36% ± 15) k_s: 0.07 ± 0.04	82 ± 24
H63A	0.0896 ± 0.028	k_f: 0.3 ± 0.14 (30% ± 2) k_s: 0.04 ± 0.01	109 ± 8
H89A	0.114 ± 0.037	k_f: 0.92 ± 0.27 (27% ± 0) k_s: 0.08 ± 0.02	80 ± 9
H63A/H89A	0.234 ± 0.072	k_f: 2.01 ± 1.15 (28 ± 9) k_s: 0.08 ± 0.056	n/a
WT	0.082 ± 0.16	k_f: 0.77 ± 0.57 (22% ± 6) k_s: 0.05 ± 0.01	126 ± 13
H64Y/V58F-Mb + Ferric Heme	1.8 (ferric heme binding)	k_f: 4.5 (42%) k_s: 0.13 (ferric heme binding)