Table 3.

The Co²⁺ selectivity over Zn²⁺ or Pb²⁺ for constructs 11BNick1–6. The effect of the alternative truncation platform involving a nick in the 5’-substrate binding arm is examined. Metal ion selectivities and pseudo-first-order rate constants for each construct are shown.

Construct		kobs [min−1]		Co:Zn	Co:Pb
	Co2+	Zn2+	Pb2+
11B	0.088±0.006	0.15±0.01	0.079±0.016	0.59±0.06	1.1±0.2
11BNick1	0.042±0.004	0.075±0.019	0.076±0.021	0.56±0.15	0.55±0.16
11BNick2	0.010±0.002	0.0073±0.0008	0.0041±0.0004	1.4±0.3	2.4±0.5
11BNick3	0.027±0.004	0.038±0.010	0.016±0.002	0.71±0.22	1.7±0.3
11BNick4	0.015±0.002	0.019±0.003	0.010±0.002	0.79±0.14	1.5±0.3
11BNick5	0.18±0.01	0.27±0.04	0.16±0.04	0.67±0.11	1.1±0.3
11BNick6	0.18±0.01	0.28±0.01	0.16±0.05	0.64±0.03	1.1±0.3