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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Jan 21;259(3):329–337. doi: 10.1016/j.taap.2012.01.010

Fig. 6. Association of mitochondrial stress and intracellular free calcium ions in NaF-mediated reduction of cell viability.

Fig. 6

(A) The relative percentage of NaF-induced MMP loss was calculated by monitoring the DiOC6 fluorescence intensity shift between the experimental and control values using WinMDI 2.9 (n = 4). (B) Mitochondrial and (C) cytosolic fractions were prepared from cells exposed to the indicated NaF concentrations for 24 h and then analyzed by western blotting. Cells were also incubated with 2 mM NaF at the indicated concentrations of BAPTA-AM (0–10 μM) or 2,500 U/ml CAT, and then (D) cell viability and (E) JNK activity were determined at 24 h and 12 h after the incubation, respectively. *p < 0.05 and **p < 0.01 vs. the untreated controls.