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. Author manuscript; available in PMC: 2013 Mar 9.
Published in final edited form as: Mol Cell. 2012 Feb 16;45(5):629–641. doi: 10.1016/j.molcel.2011.12.036

Figure 6. TECPR1 is required for autophagosome maturation.

Figure 6

(A) GFP-mRFP-LC3 was expressed and detected 48 hours after transfection in control cells or TECPR1 depleted U2OS cells treated with EBSS medium for one hour or mock treated. Scale bar (10 μm). (B) Quantification result of at least 200 cells from three independent experiments described in (A). Data are presented as means ± SD. AL, autolysosomes; AV, autophagic vacuoles. (C) The autophagic flux was examined in control and TECPR1 depleted U2OS cells mock treated (N), BafA1 or CQ treated. The intensity of LC3II/Tubulin was calculated as shown. See also Figure S6.