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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Anal Biochem. 2012 Jan 28;423(1):178–183. doi: 10.1016/j.ab.2012.01.017

Fig. 5. Competition Fluorescence Spectroscopy.

Fig. 5

Titration of unlabeled DNA hairpin into 30 nM 2:TAMRA-labled DNA. Conditions: 10 mM HEPES, 50 mM NaCl, 1 mM EDTA, pH 7.4, 25°C. Data were fit to yield a Kd of 1.1 ± 0.2 nM for the unlabeled DNA.