. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Mamm Genome. 2011 Nov 20;23(3-4):250–258. doi: 10.1007/s00335-011-9368-9

Table 1.

PCR primers and conditions for amplification from cDNA

Gene^a	Primer 1	Primer 2	Anneal	Conditions^b

2010109I03Rik	5' CTGCTTGCTCTCATTGCCCA	5' TTCAAGAAGTGCCCCACCCA	62°C	45/45/75 × 45
Ly6e	5' TCTTTGGCTTGCGAACCTTCA	5' CTTTGCCCACCCCAGAATCC	66°C	30/30/60 × 40
Ly6i	5' TTGGATGGCTGAGCCGTGTG	5' GGGTGGGGACCATCACATCA	66°C	30/30/60 × 40
Ly6a	5' GCAACCTGGTCAGAGAGGAA	5' GGAGAACAAAGGGTTTATTGGA	60°C	30/30/60 × 40
Ly6c1	5' TGGTGTCAGGAGGGAGCTGCTA	5' GGCATTTACCAAGCAGGGGC	66°C	30/30/60 × 40
Ly6c2	5' TGAGGATGGACAGTACTCACG	5' GAGTGGTTGCACATGAGGAT	60°C	30/30/30 × 40
I830127L07Rik	5' AGGACTGGTGTCAGGAGGGA	5' GCCAGGAATTGCAAGCCACA	60°C	30/30/60 × 45
Ly6g	5' GGTCAGAGAGGAACCCTTCTCC	5' GGGTGCCTATACAGCAGGGGTT	60°C	30/30/60 × 45
BC025446	5' GCCCAGGGATGTGATTGGTG	5' CCAGCACCTTGGCCACATTC	60°C	30/30/60 × 40
Ly6h	5' AGCCAAAGCTCTTCCGAGAATCTG	5' CAACAGGCTTAGGGGACAAGCTC	67°C	45/45/75 × 45
Gpihbp1	5' AGCCTGAGCCAGGATGAAGG	5' GCGTCCTGGTTGGAGGAGAA	65°C	30/30/60 × 40

Unique transcripts could not be amplified for Ly6f, Gm10238, or 9030619P08Rik with ≥ 2 primer sets (data not shown)

PCR reaction conditions listed as follows: denature(sec)/anneal(sec)/extension(sec) × number of cycles