Fig. 10.

C. albicans hyphae and yeast recovered from a murine model of oral candidiasis and immunolabeled with anti-Als antibodies. Immunosuppressed mice were inoculated orally with C. albicans CAI12 (see Materials and methods). After 5 days of infection, mice were euthanized and the tongue and oral mucosa homogenized. C. albicans cells were collected using a cell strainer, washed, fixed in paraformaldehyde, and immunolabeled with anti-Als mAbs. Cells were imaged using an Olympus BX50 FluoView microscope. (a). Anti-Als3 labeled hyphae, but not yeast cells. The left panel was illuminated with laser (488 nm) and the right with laser and white light. (b and c). Anti-Als1 (center panels) labeled both hyphae and yeast. Als4-positive hyphae (left panels) were more difficult to find compared to anti-Als1-labeled hyphae. In contrast to the restricted localizations of Als4 and Als1 on hyphae from culture, the proteins were detected over greater lengths of the hyphae recovered from the mouse model. (d and e). Anti-Als4 (left) and anti-Als1 (second from left) immunolabeled yeast cells. In many instances, anti-Als4 and anti-Als1 labeled the entire surface of the same yeast cell. Previous in vitro analyses demonstrated that anti-Als1 labels the yeast cell surface, with the exception of the bud scar (Coleman et al., 2010). In vivo, cells with a lack of labeling at one pole were detected (see arrow in d) although far more cells appeared to have an entire surface covered with anti-Als1 (e).