Figure 6. Funnel plot analysis of publication bias in studies that have evaluated the effects of DR on mouse lifespan (including ILSXISS strains).

The funnel plot analyses shown in Figures 3C and 3D were repeated using survival data from 72 mouse experiments in combination with 121 experiments that had evaluated ILSXISS recombinant inbred mouse strains (193 experiments total; Supplemental Table 2) (Liao et al., 2010a; Rikke et al., 2010). Experiments for which DR decreased median or maximum lifespan are plotted within the grey region in each figure. The dotted vertical line denotes the average effect size among all experiments (i.e., average treatment effect weighted by study size). In the absence of publication bias, it is expected that effect sizes from individual experiments will be symmetrically distributed to the left and right of the average effect size (dotted line).