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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biomed Microdevices. 2012 Apr;14(2):247–257. doi: 10.1007/s10544-011-9602-y

Fig. 3.

Fig. 3

Immobilization of GST-GFP in 5% PEG700DA hydrogels with 0–20% PEG3400 porogen. (a) Phase-contrast images of hydrogel pillars. Pillars were incubated with 2 μg/μL GST-GFP and subsequently incubated with anti-GST and Alexa Fluor 594-conjugated secondary antibody. Fluorescence images showing (b) GST-GFP and (c) anti-GST with Alexa Fluor 594-conjugated secondary antibody localization throughout the pillars. The first three columns are triplicates and pillars shown in the last column were quenched with 0.5 M hydroxylamine prior to exposure to GST-GFP. Contrast is enhanced in image to emphasize low-signal areas. (d) Fluorescence intensity of GST-GFP and anti-GST relative to those detected in hydrogels lacking porogen. Error bars = SD (n ≥ 4 pillars). (e) Typical GST-GFP and (f) anti-GST fluorescence intensity profiles along a diameter of the hydrogels pillars. Gray value range = 0–255.