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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biomed Microdevices. 2012 Apr;14(2):247–257. doi: 10.1007/s10544-011-9602-y

Fig. 5.

Fig. 5

Phosphorylation of 0–10 μg/μL GST-Crkl immobilized in 5% PEG700DA/20% PEG3400 hydrogel pillars by K562 cell lysates. (a) Anti-phosphotyrosine and anti-GST signal as a function of the concentration of GST-Crkl used for immobilization after incubating with 1 μg/μL K562 cell lysate for 1 h and labeling with antibodies. (b) Anti-phosphotyrosine signal is dependent on the anti-GST signal. Error bars = SD (n = 5 spots).