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. Author manuscript; available in PMC: 2013 Jan 5.
Published in final edited form as: Oncogene. 2011 Nov 14;31(27):3265–3276. doi: 10.1038/onc.2011.504

Figure 2.

Figure 2

Knockdown of TR3 inhibits cell growth and induces apoptosis in NSCLC cells. (A) Cell survival. After transfection with either siScr or siTR3 for 4 days, the number of cells in each well was counted. The experiment was repeated three times with four replicates each and the data are presented as means with SD. #P<0.001 vs siScr. (B-D) Cells were transfected with an indicated siRNA for 72 hours, and whole cell lysates were analyzed by Western blot analysis. β-Actin was used as a loading control and the experiment was repeated three times with similar results. TR3 knockdown (C) varied between 60-80% in this study and similar results were observed in subsequent experiments using siTR3.