Effects of EGb 761 and its components on neuronal survival in vitro. Cells were grown in 24-well plates for 14 days and then pretreated with EGb 761 (100 μg), BB (12 μg), GA (12 μg), GB (12 μg), TFM (20 μg), or vehicle control. Then they were exposed to (A) 60 M t-BuOOH, (B) 100 M H2O2, and (C) 100 M NMDA. Cell viability was assessed with the MTT assay and is shown as percent of control. Each experiment was conducted in triplicate and repeated four times with different primary culture batches. Data are expressed as mean ± SEM; #p 0.05 vs. control, *p 0.05 vs. stressor.