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. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Neurobiol Dis. 2012 Jan 24;46(1):180–189. doi: 10.1016/j.nbd.2012.01.006

Fig. 3 — EGb 761 protects neurons from apoptosis. Cultured cortical neurons from mice were pretreated for 6 h with vehicle or 100 μg/ml EGb 761 in the presence or absence of SNPPIX; then 60 μM t-BuOOH was added for an additional 18 h. (A) Immunocytochemical staining shows DAPI (blue), apoptotic cells (green), HO1 expression (red), and the overlay of all. Scale bar = 10 μm (B) The EGb 761-induced reduction in apoptosis was blocked by SNPPIX, indicating that the neuroprotection requires HO1. Each experiment was conducted in triplicate and repeated three times with different primary culture batches. Data are expressed as means ± SEM; p 0.05 vs. t-BuOOH; ^#p 0.05 vs. EGb 761+t-BuOOH.

Fig. 3 — EGb 761 protects neurons from apoptosis. Cultured cortical neurons from mice were pretreated for 6 h with vehicle or 100 μg/ml EGb 761 in the presence or absence of SNPPIX; then 60 μM t-BuOOH was added for an additional 18 h. (A) Immunocytochemical staining shows DAPI (blue), apoptotic cells (green), HO1 expression (red), and the overlay of all. Scale bar = 10 μm (B) The EGb 761-induced reduction in apoptosis was blocked by SNPPIX, indicating that the neuroprotection requires HO1. Each experiment was conducted in triplicate and repeated three times with different primary culture batches. Data are expressed as means ± SEM; p 0.05 vs. t-BuOOH; ^#p 0.05 vs. EGb 761+t-BuOOH.