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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Hepatology. 2012 Mar 20;55(5):1356–1368. doi: 10.1002/hep.25504

Figure 5. Plasma lipoprotein characterization.

Figure 5

L-MTP−/− mice were transduced with different titers of adenoviruses. After 3 days, mice were fasted (4 h) and injected with P407. Plasma was collected after 2 h, pooled, and subjected to FPLC. Triglyceride (A), cholesterol (B) and phospholipid (C) distribution in plasma lipoproteins of Ad-hMTP (left panel) and Ad-dMTP transduced mice (right panel). (D–E) VLDL fractions after FPLC were pooled and concentrated. Similarly, HDL fractions were pooled and apoAI-lipoproteins were removed by immunoprecipitation. VLDL and ApoAI-free HDL were subjected to negative staining and electron microscopy (D). VLDL Bars = 180 nm, HDL Bars = 72 nm. Average diameter of VLDL particles in different groups (Mean ± SEM, n=200–300) (E). ***p < 0. 001, **p < 0. 01, *p < 0. 05.