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. Author manuscript; available in PMC: 2013 Mar 7.
Published in final edited form as: Cell Metab. 2012 Mar 7;15(3):311–323. doi: 10.1016/j.cmet.2012.01.020

Figure 4. Klf15 regulates rhythmic nitrogeneous waste excretion.

Figure 4

Schematic illustrates the excretion of nitrogenous waste products, i.e., “the urea cycle.” (a, b) Plasma glutamate, ammonia in WT and Klf15-null mice (n=5 per group per time point). (c) Otc expression in WT and Klf15-null livers (n=4 per group per time point). (d) Plasma ornithine in WT and Klf15-null mice (n=5 per group per time point). (e) OTC enzymatic activity measured from liver mitochondrial extracts from WT and KLF15-null mice (n=4 per group). (f, g) Urinary levels of urea and ammonia in WT and KLF15-null mice (n=5 per group). (h) ChIP for KLF15 on the Otc promoter (n=3 per time point). (# p<0.05 at all time points between WT and Klf15-null). (i–k)Results of neurobehavioral testing for (i) Y-maze, a test of working memory (n=3 per group) (j) Fear conditioning during contextual changes (hippocampal function) and altered cues (amygdalar function) (n=8 per group) and (k) Morris water maze test, a test of hippocampal function (n=8 per group). Data presented as mean ± SEM.