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Identification, by thin-layer chromatography, of the base methylated by the Hia5, Hin1523 and Nme1821 enzymes
| Enzyme |
3H incorporation (dpm) |
|||
|---|---|---|---|---|
| Solvent Ga |
Solvent Db |
|||
| m6dA | m4dC + m5dC | m6dA + m4dC | m5dC | |
| Hia5 | 5820 | 27 | 5700 | 22 |
| Nme1821 | 5670 | 20 | 5595 | 20 |
| Hin1523 | 5033 | 17 | 5001 | 25 |
am4dC and m5dC comigrate in solvent G. bm6dA and m4dC comigrate in solvent D.
