Table 4.
Percentages of the base content of λ DNA (control or methylated with Hia5, Hia5D194A or Hin1523 proteins) and total DNA of H. influenzae biotype aegyptius ATCC 11116 as determined by the HPLC DNA methylation assay
| DNA | dC | dmC | dG | dT | dA | dmA | dmA/ΣA | %GC |
|---|---|---|---|---|---|---|---|---|
| λ dam− dcm− | 23.60 | 0.00 | 26.10 | 25.30 | 25.00 | 0.00 | 0.0 | 49.70 |
| λ dam+ dcm+ | 23.60 | 0.04 | 25.88 | 24.58 | 25.78 | 0.12 | 0.46 | 49.52 |
| λ methylated by Hia5 | 23.90 | 0.00 | 25.40 | 24.00 | 10.20 | 16.50 | 61.79 | 49.30 |
| λ methylated by Hia5D194A | 25.00 | 0.00 | 25.10 | 24.60 | 25.20 | 0.00 | 0.00 | 50.20 |
| λ methylated by Hin1523 | 23.40 | 0.00 | 24.90 | 24.70 | 19.00 | 8.00 | 29.63 | 48.30 |
| Total DNA of H. influenzae biotype aegyptius ATCC 11116 | 21.00 | 3.70 | 19.20 | 26.80 | 28.80 | 0.50 | 1.70 | 43.90 |
ΣA = dA + dmA. Values presented are the means obtained using several independent DNA preparations.