Table 1.
Escherichia coli bacterial strains and plasmids used in this work
| Strains or plasmids | Relevant genotype | References |
|---|---|---|
| Strains | ||
| SR-14 | Δ7 rrn Δ(lacZYA) ΔrecA λ tnap tnaC(tnaA’-‘lacZYA) (prrnC-sacB, ptRNA67) | (19) |
| SQ351 | MG1655 Δ7 rrn Δ(lacZYA) (pKK3535, ptRNA67) | (19) |
| AW122 | Derived from SQ351 (prrnC-sacB, ptRNA67) | This work |
| AW182 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(tnaA’-‘lacZYA) (prrnC-sacB, ptRNA67) | This work |
| AW216 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(tnaA’-‘lacZYA) (pNK, ptRNA67) | This work |
| AW218 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(tnaA’-‘lacZYA) (pKKU2609C, ptRNA67) | This work |
| AW221 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(W12R)(tnaA’-‘lacZYA) (pNK, ptRNA67) | This work |
| AW227 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(tnaA’-‘lacZYA) (pNH153, ptRNA67) | This work |
| AW326 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(K18A)(tnaA’-‘lacZYA) (pNK, ptRNA67) | This work |
| AW600 | MG1655 Δ7 rrn Δ(lacZYA) att7::tnaptnaC(D16A)(tnaA’-‘lacZYA) (pNK, ptRNA67) | This work |
| Plasmids | ||
| pNK | Wild-type rrnB operon; Ampr, derived from ColE1 | (13) |
| pNH153 | Derived from pNK; has an insertion at position 751 in the 23S rRNA gene | (13) |
| pKKU2609C | Derived from pNK; has a T-to-C replacement at position 2609 in the 23S rRNA gene | (33) |
| pNKA752C | Derived from pNK; has a A-to-C replacement at position 752 in the 23S rRNA gene | (36) |
| pK4–16 | Wild-type rrnB operon; Kmr, derived from SC101 | Quan and Squires, personal communication |
| prrnC-sacB | Wild-type rrnC gene and a sacB gene, derived from SC101 | (45) |
| pKK3535 | Wild-type rrnB operon, derived from pBR322 | (37) |
| ptRNA67 | tRNA encoding plasmid | (45) |
| pGF2500 | Wild-type tnaC gene with the rpoBC terminator, derived from pUC18 | (5) |
| pAW137 | Has the tnaptnaC(ΔN2-H22) with BsaI–XhoI–BsaI linker-tna-'-'lacZYA cloning reporter gene, derived from pACYC184 | This work |