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Single pot reaction to generate small RNA pools. (A) Three ribonucleases were tested for the ability to generate siRNA pools during in vitro transcription. Reactions contained either 275 ng/ul human Dicer, 0.01 unit/µl ‘ShortCut’ RNase III, or 10 ng/µl Giardia Dicer. RNA products were resolved by denaturing PAGE and stained with ethidium bromide. (B) Schematic of the optimized method for producing small RNAs using Giardia Dicer.
