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. 2011 Dec 19;40(5):e37. doi: 10.1093/nar/gkr1251

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Validation of vanillic acid-responsive promoter variants containing different numbers of VanO operator modules. Vectors encoding SEAP expression driven by a vanillic acid-responsive promoter harbouring monomeric (pMG262), dimeric (pMG252), trimeric (pMG263) or tetrameric (pMG264) operator modules were co-transfected with pMG250 (P_SV40-VanA₁-pA) into (A) CHO-K1, (B) HEK-293 and (C) BHK-21 cells and SEAP production was scored after cultivation for 48 h in the presence and absence 250 µM vanillic acid.