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. 2011 Dec 19;40(5):e37. doi: 10.1093/nar/gkr1251

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Combinatorial validation of the VAC_OFF system in different transactivator and promoter configurations. VAC_OFF transactivators employing different transactivation domains (A: VanA₁, VanR-VP16; pMG250) (B: VanA₂, VanR-p65; pMG256) (C: VanA₃, VanR-E2F4; pMG257) were co-transfected with different vanillic acid-responsive promoter variants containing 0 (P_1VanO2; pMG252), 2 (P_2VanO2; pMG265), 4 (P_3VanO2; pMG266), 6 (P_4VanO2; pMG267), 8 (P_5VanO2; pMG268) and 10 (P_6VanO2; pMG269) base-pair linkers between VanO and the minimal promoter into CHO-K1 cells. All promoter variants drove SEAP expression and the production was profiled 48 h after cultivation of the cells in media containing different concentrations of vanillic acid (0, 50 and 250 μM).