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. 2011 Dec 20;40(5):e39. doi: 10.1093/nar/gkr1264

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Selected evolution of FPBA. (A) Aptamer sequences isolated from the first stage of SELEX. All 36 selected sequences of the 50 nt randomized region of the full length isolates comprised two families of highly related sequences. Varying residues within individual families are underlined. The first aptamer of each family was used in nitrocellulose filter-binding assays in Part B. (B) Radioactive (P³²) aptamer binding to GFP immobilized on nitrocellulose demonstrates the higher affinity of G16 relative to the G3 family. (C) Aptamer sequences selected following a second stage of SELEX in which the fixed regions flanking the G16 core were optimized. About 11 other single copy sequences from a total 36 clones were not shown. The flanking sequences shared by all selected molecules are underlined. Note the shorted 3′ sequences of AP2 and AP3. (D) The nitrocellulose filter-binding assay shows the higher affinity of the selected aptamers relative to G16.