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. 2011 Nov 15;40(5):2294–2311. doi: 10.1093/nar/gkr765

Figure 3.

Figure 3.

Phenotypic and biochemical analysis of i/TIF34 and b/PRT1 mutations that disrupt subunit interactions. (A) The prt1-W674A and -YR/AA but not -W674F mutations produce severe slow growth and temperature sensitive phenotypes. The YAH06 (prt1Δ) strain was transformed with the corresponding plasmids carrying individual mutant alleles and the resident pCR52 (PRT1,URA3) covering plasmid was evicted on 5-FOA. The resulting strains were then spotted in four serial 10-fold dilutions on SD medium and incubated at 30, 34 and 37°C. (B) The tif34-DD/KK, -L222D and L222K mutations produce severe slow growth and temperature sensitive phenotypes. The H450 (tif34Δ) strain was transformed with the corresponding plasmids carrying individual mutant alleles and the resident YEp-i/TIF34-U (TIF34, URA3) covering plasmid was evicted on 5-FOA. The resulting strains were then spotted in four serial 10-fold dilutions on SD medium and incubated at 30, 34 and 37°C. (C) Summary of phenotypes of mutations analyzed in this study.