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. 2011 Nov 3;40(5):2140–2151. doi: 10.1093/nar/gkr894

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Extraction of both thermodynamic and kinetic parameters from a single binding titration. Forward titration with titrant [TT] = 229 µM, starting cell [RR] = 10 µM, using 5 µL injections, at 20°C in 0.5 mM MgCl₂ solution. (A) Raw power trace following baseline subtraction to present excess power [E = (∂Q/∂t)]. (B) Binding curve following integration of each peak to plot the concentration-normalized heat evolved (Q_norm) against the current molar ratio. Best fit parameters for the displayed dataset are listed. (C) Injection Peak 2 following deconvolution (E_dc); best-fit curve and resulting k_on value for the peak using fit methodology as described in ‘Materials and Methods’ section are provided.