Figure 6.

Co-transcriptional assemblies of tectoRNA attenuators 1, 4, 7 and 9 in presence (or absence) of cognate GAAA probe. (A) Schematic illustrating the possible molecular states adopted by the tectoRNA attenuator system during its transcription from DNA templates (in blue) by T7 RNA polymerase (in green) at 37°C in presence of 10 mM Mg²⁺. (B) Native PAGE analysis of different tectoRNA attenuator transcription mixtures at various times in presence (+) or absence (−) of GAAA probe: co-transcriptional assembly is monitored by RNA body-labeling with α[P³²]ATP and native PAGE is performed at 10°C and 10 mM Mg(OAc)₂ after quenching the transcription with DNase as described in the ‘Materials and Methods’ section. See also Supplementary Figure S5.