Figure 1.

Somatic mutation detection using single cell sequencing. (A) Somatic mutations in tissues are rare and therefore found only in single sequencing reads from which they are routinely filtered out as sequencing errors during post-alignment processing. Adopting a single cell approach overcomes this limitation by transforming each somatic event into a consensus variant call. (B) Schematic depiction of the single cell sequencing protocol used for Drosophila S2 cells.