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. 2012 Feb 28;40(5):2345–2356. doi: 10.1093/nar/gkr970

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Quantitative fluorescence analysis of β-turn substitution on Py–Im polyamide nuclear uptake. (A) Nuclear localization of β-aryl polyamide 13, as verified by colocalization with Hoechst 33342. (B) Influence of incubation time on fluorescence for A549 cells treated with 100 nM polyamide 13 or 14. X-axis: relative median fluorescence (FL1: FITC channel); Y-axis: hours of polyamide treatment. (C) Influence of dosage concentration on nuclear accumulation of polyamides. Polyamide 13 concentration: 100, 1000, 10 000 nM. Polyamide 14 concentration: 100, 1000 nM.