Figure 4.

NuA4 HAT is required for recruitment of the TFIID complex to the RPS5 promoter. (A) Analysis of the recruitment of TBP and TAFs components of the TFIID complex to the RPS5 core promoter in the esa1-ts mutant and its isogenic wild-type equivalent. Yeast cells were grown as in Figure 3C, but Esa1p was inactivated for 1 h at the non-permissive temperature. Immunoprecipitation was performed using anti-TBP, anti-TAF1 and anti-TAF12 antibodies against TBP, TAF1 and TAF12 (obtained from the Green laboratory; 13). (B) RT–PCR analysis of RPS5 and ACT1 transcripts in the esa1-ts mutant and its isogenic wild-type equivalent following 1 h ts inactivation at the non-permissive temperature.