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. 2011 Nov 15;40(5):1969–1983. doi: 10.1093/nar/gkr977

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — The 19S base enhances the targeting of NuA4 HAT, but not activator Rap1p, to the promoters of RPL2B and RPS11B. (A) Analysis of recruitment of the 19S base (Rpt2p-myc) to the promoters of RPL2B and RPS11B. Yeast cells were grown, cross-linked and immunoprecipitated as in Figure 1B. (B) Analysis of recruitment of NuA4 HAT (Esa1p-myc) to the promoters of RPL2B and RPS11B. Yeast cells were grown, cross-linked and immunoprecipitated as in Figure 3B. (C) Analysis of recruitment of NuA4 HAT (Esa1p-myc) to the promoters of RPL2B and RPS11B in the rpt4-ts mutant and its wild-type equivalent following 1 h ts inactivation at the non-permissive temperature. (D) Analysis of recruitment of Rap1p to the promoters of RPL2B and RPS11B in the rpt4-ts mutant and its wild-type equivalent following 1 h ts inactivation at the non-permissive temperature.