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. 2011 Nov 15;40(5):2089–2106. doi: 10.1093/nar/gkr983

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Mph1 DNA helicase activity on four-way junction substrates. (A) Left panel: the structure of the partially movable four-way junction substrate (containing 10-bp core of homology). Dotted lines indicate homology core and each four arm is named as N (North), S (South), E (East) and W (West) as indicated. Right panel: Branch migration activity of Mph1 as a function of time (3, 20 and 60 min). Reactions were performed with 120 fmol of Mph1 in 10 μl reaction volume. The amount of product formed was measured and indicated at bottom of the panel. (B) Branch migration activity of Mph1 in the presence of various ribo- and deoxyribonucleotides.