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. 2011 Nov 15;40(5):2089–2106. doi: 10.1093/nar/gkr983

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Mph1 helicase activity on regressed lagging or leading strand replication fork substrate. (A) Mph1 helicase activity on regressed lagging strand replication fork substrate. Assays were performed with 60 fmol of Mph1 using standard reaction condition (20 μl reaction volume) for various incubation periods (0, 1, 2, 5, 15 and 30 min). The amount of product formed was plotted against the time of incubation. (B) Assays were performed with same substrate as in (A), but labeled differently as illustrated. (C) Mph1 helicase activity on regressed leading strand replication fork substrate. Assays were performed as in (A) and the amount of product formed was plotted against incubation time. (D) Assays were performed with same substrate as in (C), but labeled differently as illustrated. (E) The mechanism of Mph1 unwinding of regressed replication fork substrates are shown based on the results from (A–D). Arrows with solid line indicate the preferred pathway while dotted lines denote the minor pathways.

Figure 9. — Mph1 helicase activity on regressed lagging or leading strand replication fork substrate. (A) Mph1 helicase activity on regressed lagging strand replication fork substrate. Assays were performed with 60 fmol of Mph1 using standard reaction condition (20 μl reaction volume) for various incubation periods (0, 1, 2, 5, 15 and 30 min). The amount of product formed was plotted against the time of incubation. (B) Assays were performed with same substrate as in (A), but labeled differently as illustrated. (C) Mph1 helicase activity on regressed leading strand replication fork substrate. Assays were performed as in (A) and the amount of product formed was plotted against incubation time. (D) Assays were performed with same substrate as in (C), but labeled differently as illustrated. (E) The mechanism of Mph1 unwinding of regressed replication fork substrates are shown based on the results from (A–D). Arrows with solid line indicate the preferred pathway while dotted lines denote the minor pathways.