Figure 6.
Precise mapping of a region of vRNA 7 interacting with vRNA 6. (A) A region interacting with vRNA 6 was identified using oligonucleotides complementary to the 3′ and 5′ regions of vRNA 7. A representative gel is shown. Ethidium bromide (EtBr) staining allowed quantification of the complex formed between vRNAs 6 and 7, while autoradiography of the 32P radiolabelled oligonucleotides allowed to monitor binding of the DNA oligonucleotides to vRNA 7 during in vitro transcription. (B) A deletion or substitutions in the 918–940 region of vRNA 7 affect interaction with vRNA 6. Mutation S71–R73 correspond to substitution of nucleotides 924, 925 926, 930 and 932. In A and B, intermolecular complexes are marked by asterisks. Relative variations of the amount of complex were determined as in Figure 4 and are given as mean ± SEM (n = 3−7).