Figure 4. The αE175R mutation has equivalent effects in each α subunit.

AChRs were expressed having 0 (wt), 1 (R_hyb) or 2 (R) mutated α subunits. A, low time resolution view of current clusters. B, left, R–E analysis of cluster populations. Right, example currents at higher resolution. Only a single hybrid population was apparent, with a fold-change in E₂ (relative to the wt) that was half of that for the double-mutant.