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. 2011 Nov 10;23(3):302–310. doi: 10.1089/hum.2011.086

FIG. 2.

FIG. 2.

Whole mounts of auditory epithelium from the basal (a, b, d) and second (c) cochlear turn after HA-assisted Ad-eGFP inoculation via the RWM. eGFP-positive cells (green) indicate transduction, and phalloidin (red) was used to stain F-actin. (axy) Confocal images showing outer hair cells (OHC), Deiters' cells (arrowheads), and pillar cells (P) with robust eGFP expression. (axz) Tangential optical section through the first row of outer hair cells showing eGFP expression. (ayz) A radial section showing an eGFP-positive outer hair cell (*) adjacent to a pillar cell (P). (b) Confocal stack of images showing eGFP expression in interdental cells (IDC) in basal turn. (c) Epifluorescence image showing the second turn organ of Corti with intact cells and normal organization pattern and a transduced inner sulcus cell. (d) Epifluorescence for actin (d), eGFP (d′), and merged (d″) showing eGFP in Deiters' cells (arrowheads) and pillar cells (arrows). Scale bars=50 μm in a–c and 40 μm in d.