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. 2011 Nov 10;23(3):302–310. doi: 10.1089/hum.2011.086

FIG. 3.

FIG. 3.

Transgene expression of eGFP after HA-assisted Ad-eGFP inoculation via the RWM (a, c–h) or Ad-eGFP inoculation via cochleostomy (b) in cells lining the scala tympani. (a) Mesothelial cells and fibrocytes with eGFP expression, third turn. (b) No eGFP expression was observed in ears with Ad-eGFP inoculated via cochleostomy (no HA) in upper turns (third turn shown here). (c) Low-magnification image of the stria vascularis and spiral ligament. (d) Light microscopy image of (c). (e) Reissner's membrane (RM) shows eGFP expression in the basal turn. (f) Mesothelial cells line the scala tympani; focal plane is lower than the luminal surface of the organ of Corti. (g) Connective tissue in spiral ligament shows eGFP expression, third turn. (h) Fibrocytes within the osseous spiral lamina with eGFP expression, second turn. P, pillar cells; IDC, interdental cells; SPL, spiral ligament; SV, stria vascularis. Scale bars=50 μm for all images (paired images are at same scale).