Figure 3.

Bim affects cytokine secretion differentially through homeostasis-driven or alloantigen-induced proliferation. Lethally irradiated Thy1.1 (Ly5.1^-) (syngeneic) or BALB/c (allogeneic) recipients were infused with B6 purified WT (Ly5.1⁺) or Bim^-/- (Ly5.1^-) T cells. Four days after cell transfer, recipient splenocytes were analyzed for expression of surface markers and intracellular cytokines that were measured in splenocytes from allogeneic (A) or syngenic (C) recipients. The absolute number of IFN-γ and TNF-α producing cells were shown in the spleen of allogeneic (B) or syngenic (D) recipients. Results shown are representative of three replicate experiments with 3 mice per group.