Figure 2.

Aneuploidy in cultured hematopoietic cells is associated with oxidative stress. LSK cells that are enriched with HSCs and LK cells (myeloid progenitors) were sorted from freshly harvested bone marrow cells. A. LSK cells and LK cells sorted were cultured under normoxic (20% oxygen) or hypoxic (3% oxygen) conditions for two days. Karyotypic analyses were performed as described in Materials and methods. B. Whole bone marrow cells were cultured under normoxic or hypoxic conditions for one and three days. Karyotypic analyses for non-megakaryocytes were performed as described above. C. LK cells sorted or whole bone marrow cells were cultured in the presence of the antioxidant N-Acetyl-Cysteine (NAC) (0.1 μM) for two days. Karyotypic analyses were performed as described above. D. LK cells sorted were cultured in the presence of the antioxidant N-Acetyl-Cysteine (NAC) at the indicated concentrations for two days. Karyotypic analyses were performed as described above. For all the experiments, at least 100 metaphase spreads were counted for each group of each cell type.